Chinese Journal of Traumatology, Vol 16, No 3 (2013)

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Influence of acute ethanol intoxication on neuronal apoptosis and Bcl-2 protein expression after severe traumatic brain injury in rats

HE Min, LIU Wei-guo, WEN Liang, DU Hang-gen, YIN Li-chun, CHEN Li


【Abstract】Objective: To study the influence and
mechanism of acute ethanol intoxication (AEI) on rat neu-ronal apoptosis after severe traumatic brain injury (TBI).
Methods: Ninety-six Sprague-Dawley rats were ran-domly divided into four groups: normal control, AEI-only,
TBI-only and TBI+AEI (n=24 for each). Severe TBI model
was developed according to Feeney’s method. Rats in
TBI+AEI group were firstly subjected to AEI, and then suf-fered head trauma. In each group, animals were sacrificed at
6 h, 24 h, 72 h, and 168 h after TBI. The level of neuronal
apoptosis and the expression of Bcl-2 protein were deter-mined by TUNEL assay and immunohistochemical method,
Results: Apoptotic cells mainly distributed in the cortex and white matter around the damaged area. Neuronal
apoptosis significantly increased at 6 h after trauma and
peaked at 72 h. Both the level of neuronal apoptosis and
expression of Bcl-2 protein in TBI-only group and TBI+AEI
group were higher than those in control group (P<0.05).
Compared with TBI-only group, the two indexes were much
higher in TBI+AEI group at all time points (P<0.05).
Conclusion: Our findings suggest that AEI can increase neuronal apoptosis after severe TBI.
Key words: Craniocerebral trauma; Apoptosis; Alcoholism

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