Xu-Kai Yang,Nan Wang,Cheng Yang,Yang-Min Wang,Tuan-Jie Che.[J].中华创伤杂志英文版,2018,21(6):316-322
Differential protein expression in patients with urosepsis
  
DOI:10.1016/j.cjtee.2018.07.003
KeyWord: Protein expressionUrosepsisProteomics
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Author NameAffiliation
Xu-Kai Yang Department of Urology, Lanzhou General Hospital PLA, Lanzhou 730050, China 
Nan Wang Department of Infection, Xi'an Central Hospital, Xi'an 710033, China 
Cheng Yang Student teams, Basic Medical College, The Fourth Military Medical University, Xi'an, 710032 China 
Yang-Min Wang Department of Urology, Lanzhou General Hospital PLA, Lanzhou 730050, China 
Tuan-Jie Che Lanzhou Baiyuan Gene Technology Co. Ltd, Lanzhou 730000, China 
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Abstract:
      Purpose: Urosepsis in adults comprises approximately 25% of all sepsis cases, and is due to complicated urinary tract infections in most cases. However, its mechanism is not fully clarified. Urosepsis is a very complicated disease with no effective strategy for early diagnosis and treatment. This study aimed to identify possible target-related proteins involved in urosepsis using proteomics and establish possible networks using bioinformatics. Methods: Fifty patients admitted to the Urology Unit of Lanzhou General PLA (Lanzhou, China), from October 2012 to October 2015, were enrolled in this study. The patients were further divided into shock and matched-pair non-shock groups. 2-DE technique, mass spectrometry and database search were used to detect differentially expressed proteins in serum from the two groups. Results: Six proteins were found at higher levels in the shock group compared with non-shock individuals, including serum amyloid A-1 protein (SAA1), apolipoprotein L1 (APOL1), ceruloplasmin (CP), haptoglobin (HP), antithrombin-III (SERPINC1) and prothrombin (F2), while three proteins showed lower levels, including serotransferrin (TF), transthyretin (TTR) and alpha-2-macroglobulin (A2M). Conclusion: Nine proteins were differentially expressed between uroseptic patients (non-shock groups) and severe uroseptic patients (shock groups), compared with non-shock groups, serum SAA1, APOL1,CP, HP, SERPINC1and F2 at higher levels, while TF, TTR and A2M at lower levels in shock groups.these proteins were mainly involved in platelet activation, signaling and aggregation, acute phase protein pathway, lipid homeostasis, and iron ion transport, deserve further research as potential candidates for early diagnosis and treatment. (The conclusion seems too simple and vague, please re-write it. You may focus at what proteins have been expressed and introduce more detail about its significance.)
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